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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: CMAS and ST3GAL4 Play an Important Role in the Adsorption of Influenza Virus by Affecting the Synthesis of Sialic Acid Receptors
doi: 10.3390/ijms22116081
Figure Lengend Snippet: The effect of CMAS and ST3GAL4 on the synthesis of different types of sialic acid receptors. CMAS-KO and ST3GAL4-KO NPTr cells were generated through the CRISPR/Cas9 system; NPTR-Cas9 cells were used as wild-type control. (A–C) The expression of sialic acid was analyzed by lectin staining. WT NPTr cells and KO NPTr cells were treated with lectins specific for α-2,6-linked sialic acid (SNA) or α-2,3-linked sialic acid (MAL). (A) Fluorescent microscopy analysis of the effect of CMAS or ST3GAL4 knockout on the synthesis of sialic acid receptors. (B) Flow cytometry analysis of the effect of CMAS knockout on the synthesis of sialic acid receptors. (C) Flow cytometry analysis of the effect of ST3GAL4 knockout on the synthesis of sialic acid receptors. Blank cells were stained with only Cy5-streptavidin, but not treated with lectin. Scale bar = 20 μm or 5 μm.
Article Snippet: The reagents used in experiments were DAPI (4′,6-diamidino-2-phenylindole; 1:1000) (catalog no. C1002; Beyotime, Shanghai, China); Biotinylated Sambucus Nigra (SNA) and Maackia Amurensis Lectin II (MAL II) lectins (catalog no. B-1305-2 and B-1265-1; Vector Lab, Burlingame, CA, USA) and
Techniques: Generated, CRISPR, Expressing, Staining, Microscopy, Knock-Out, Flow Cytometry